Cat#:RP-2742H;Product Name:Recombinant Human CSTA Protein, Active;Synonym:Cystatin-A, Cystatin-AS, Stefin-A, CSTA, STF1, STFA.;Description:Cystatin A Protein produced in E.coli is a single, non-glycosylated, Polypeptide chain containing 118 amino acids (1-98a.a.) and having a molecular mass of 13.1 kDa. The Cystatin A is fused to a 20 amino acid His tag at N-terminus and purified by proprietary chromatographic techniques.;Source:E.coli;AA Sequence:MGSSHHHHHH SSGLVPRGSH MIPGGLSEAK PATPEIQEIV DKVKPQLEEK TNETYGKLEA VQYKTQVVAG TNYYIKVRAG DNKYMHLKVF KSLPGQNEDL VLTGYQVDKN KDDELTGF.;Purity:Greater than 95% as determined by reducing SDS-PAGE.;Bioactivity:The IC50 value is < 1.0nM. The inhibitory function of CSTA on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C.;Formulation:Cystatin-A (1mg/ml) in 20mM Tris-HCl buffer (pH8.0), 1mM DTT and 10% glycerol.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Storage:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.? For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid repeated freeze-thaw cycles.;