Cat#:RP-2733H;Product Name:Recombinant Human CST3 Protein, Active;Synonym:Cystatin-C, Cystatin-3, Neuroendocrine basic polypeptide, Gamma-trace, Post-gamma-globulin, CST3, MGC117328.;Description:Cystatin-C Protein produced in HEK cells is a non-glycosylated monomer, having a molecular weight of approximately 13kDa. The Cystatin-C is purified by proprietary chromatographic techniques.;Source:HEK.;Purity:Greater than 95% as obsereved by SDS-PAGE.;Bioactivity:The inhibitory function of Cystatin-C on papin’s protease activity was measured by a colorimetric assay using L-BAPA as substrate. IC50 value was measured at 5-20 µg/ml (0.3-1.5 µM) with a range of 1.56-50µg/ml Cystatin-C in presence of 0.55µM papain and ;Formulation:The Cystatin-C was lyophilized from 1mg/ml in 1xPBS.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized Cystatin-C in sterile water not less than 100µg/ml, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized?Cystatin-C although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Cystatin-C should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.;