Cat#:RP-2652H;Product Name:Recombinant Human COL4A3 Protein;Synonym:Collagen alpha-3(IV) chain, Goodpasture antigen, COL4A3, Glomerular Basal Membrane, GBM.;Description:Human a3 chain of collagen IV; identical with the antigen called "glomerular basal membrane antigen" (GBM). Recombinant antigen for solid (ELISA) and fluid phase diagnostic assays. Binds IgG-type human auto-antibodies. Calculated Molecular weight: 43,591 Dalton. Calculated isoelectric point: pH 8.9. cDNA coding for a minicollagen version of the human collagen IV a3 chain fused to a hexa-histidine purification tag. The term minicollagen designates the removal of most of the epitope-less triplehelical collagenous region (situated between the N-terminal 7S domain and the C-terminal noncollagenous NC1 domain), which is a requirement for recombinant production of this antigen.;Source:Sf9 insect cells.;Bioactivity:Please contact us for detailed information;Formulation:The protein solution (0.6mg/ml) contains 20mM Hepes, pH 8.0, and 4M Urea.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Storage:Recommendations for storage buffer: Ionic strength between 50 and 100mM, neutral to slightly alkaline pH and 4M urea as dissociating agent. Storage temperature is -70° to -80° C. Please prevent freeze-thaw cycles.;
Human a3 chain of collagen IV; identical with the antigen called "glomerular basal membrane antigen" (GBM). Recombinant antigen for solid (ELISA) and fluid phase diagnostic assays. Binds IgG-type human auto-antibodies. Calculated Molecular weight: 43,591 Dalton. Calculated isoelectric point: pH 8.9. cDNA coding for a minicollagen version of the human collagen IV a3 chain fused to a hexa-histidine purification tag. The term minicollagen designates the removal of most of the epitope-less triplehelical collagenous region (situated between the N-terminal 7S domain and the C-terminal noncollagenous NC1 domain), which is a requirement for recombinant production of this antigen.
Source:
Sf9 insect cells.
Bioactivity:
Please contact us for detailed information
Formulation:
The protein solution (0.6mg/ml) contains 20mM Hepes, pH 8.0, and 4M Urea.
Stability:
Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃
Storage:
Recommendations for storage buffer: Ionic strength between 50 and 100mM, neutral to slightly alkaline pH and 4M urea as dissociating agent. Storage temperature is -70° to -80° C. Please prevent freeze-thaw cycles.