Cat#:RP-0326H;Product Name:Recombinant Human CD3D & CD3E Heterodimer Protein;Synonym:CD3-DELTA,IMD19,T3D;Description:A DNA sequence encoding the extracellular domain (Met 1-Ala 105) of human CD3D (P04234) was fused with the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met 1-Asp 126) of human CD3E (P07766) was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human CD3D/CD3E heterodimer was purified.;Source:Human Cells;Predicted N Terminal:Phe 22 & Asp 23;Molecular Characterization:The recombinant heterodimer of human CD3D/CD3E comprises 670 (333+354) amino acids and has a calculated molecular mass of 77.5 (37.5+40锛?kDa. As a result of glycosylation, the apparent molecular mass of human CD3D/CD3E heterodimer is approximately 45-50 k;Purity:Greater than 90 % as determined by SDS-PAGE;Endotoxin:< 1.0 EU per ug of the protein as determined by the LAL method;Bioactivity:Please contact us for detailed information;Formulation:Lyophilized from sterile PBS, pH 7.4;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:Please see related COA for specific instructions.;Storage:Avoid repeated freeze-thaw cycles. In lyophilized state for 1 year (4-8℃); Upon reconstitution under sterile conditions for 2 weeks (4-8℃) or 3 months (-20℃ to -70℃).;