Cat#:RP-4466H;Product Name:Recombinant Human CCL8 / MCP2 Protein;Synonym:Small inducible cytokine A8, CCL8, Monocyte chemotactic protein 2, MCP-2, Monocyte chemoattractant protein 2, HC14, chemokine (C-C motif) ligand 8, MCP2, SCYA8, SCYA10.;Description:Recombinant human CCL8 Protein expressed in E.coli is a non-glycosylated, Polypeptide chain containing 76 amino acids and having a molecular mass of 8904 Dalton. The recombinant human CCL8 protein is purified by our unique chromatographic techniques.;Source:E.coli;AA Sequence:The sequence of the first five N-terminal amino acids was determined and was found to be Gln-Pro-Asp-Ser-Val.;Purity:Greater than 95.0% as determined by RP-HPLC and SDS-PAGE.;Bioactivity:The biological activity of recombinant human CCL8 protein was determined by measuring the dose dependent mobilization of intracellular calcium (calcium flux) with human THP-1 cells. Significant calcium mobilization is observed with 500ng/mL of recombinant human MCP-2. Human MCP-2 also ind;Formulation:The recombinant human CCL8 protein was lyophilized from a concentrated (1mg/ml) sterile solution containing no additives.;Stability:Recombinant Human CCL8 Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized human CCL8 Protein-2in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized recombinant human CCL8 protein although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution recombinant human CCL8 protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.;References:Synergistic up-regulation of MCP-2/CCL8 activity is counteracted by chemokine cleavage, limiting its inflammatory and anti-tumoral effects. Struyf S, et al. Eur J Immunol, 2009 Mar. PMID 19224633;
Small inducible cytokine A8, CCL8, Monocyte chemotactic protein 2, MCP-2, Monocyte chemoattractant protein 2, HC14, chemokine (C-C motif) ligand 8, MCP2, SCYA8, SCYA10.
Description:
Recombinant human CCL8 Protein expressed in E.coli is a non-glycosylated, Polypeptide chain containing 76 amino acids and having a molecular mass of 8904 Dalton. The recombinant human CCL8 protein is purified by our unique chromatographic techniques.
Source:
E.coli
AA Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Gln-Pro-Asp-Ser-Val.
Purity:
Greater than 95.0% as determined by RP-HPLC and SDS-PAGE.
Bioactivity:
The biological activity of recombinant human CCL8 protein was determined by measuring the dose dependent mobilization of intracellular calcium (calcium flux) with human THP-1 cells. Significant calcium mobilization is observed with 500ng/mL of recombinant human MCP-2. Human MCP-2 also ind
Formulation:
The recombinant human CCL8 protein was lyophilized from a concentrated (1mg/ml) sterile solution containing no additives.
Stability:
Recombinant Human CCL8 Proteins are stable for up to 1 year from date of receipt at -70℃
Reconstitution:
It is recommended to reconstitute the lyophilized human CCL8 Protein-2in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage:
Lyophilized recombinant human CCL8 protein although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution recombinant human CCL8 protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
References:
Synergistic up-regulation of MCP-2/CCL8 activity is counteracted by chemokine cleavage, limiting its inflammatory and anti-tumoral effects. Struyf S, et al. Eur J Immunol, 2009 Mar. PMID 19224633