Cat#:RP-0216H;Product Name:Recombinant Human CASP3 / Caspase-3 / CASP-3 Protein (His Tag);Synonym:CPP32,CPP32B,SCA-1,Caspase 3 Apoptosis-Related Cysteine Peptidase, CPP32 Caspase 3 Apoptosis-Related Cysteine Protease, Cysteine Protease CPP32, Protein Yama, CASP-3, CPP-32, SCA-1, SREBP Cleavage Activity 1, EC 3.4.22.56,caspase-3, PARP Cleavage Protease;Description:A DNA sequence encoding the mature form of human caspase3 (Q5IS54) (Ser29-His277) was expressed with a polyhistidine tag at the C-terminus.;Source:E. coli;Predicted N Terminal:Met;Molecular Characterization:The recombinant human caspase3 consists of 259 amino acids and predicts a molecular mass of 29.9 KDa. It migrates as an approximately 17 and 15 KDa band in SDS-PAGE under reducing conditions.;Purity:(48.4+48.1) % as determined by SDS-PAGE;Endotoxin:Please contact us for more information.;Bioactivity:Please contact us for detailed information;Formulation:Lyophilized from sterile 20mM HEPES, 100mM NaCl, 1mM EDTA, 10% Sucrose, 0.1% chaps, pH 7.5.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:Please see related COA for specific instructions.;Storage:Avoid repeated freeze-thaw cycles. In lyophilized state for 1 year (4-8℃); Upon reconstitution under sterile conditions for 2 weeks (4-8℃) or 3 months (-20℃ to -70℃).;
A DNA sequence encoding the mature form of human caspase3 (Q5IS54) (Ser29-His277) was expressed with a polyhistidine tag at the C-terminus.
Source:
E. coli
Predicted N Terminal:
Met
Molecular Characterization:
The recombinant human caspase3 consists of 259 amino acids and predicts a molecular mass of 29.9 KDa. It migrates as an approximately 17 and 15 KDa band in SDS-PAGE under reducing conditions.
Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃
Reconstitution:
Please see related COA for specific instructions.
Storage:
Avoid repeated freeze-thaw cycles. In lyophilized state for 1 year (4-8℃); Upon reconstitution under sterile conditions for 2 weeks (4-8℃) or 3 months (-20℃ to -70℃).