Cat#:RP-2199H;Product Name:Recombinant Human Bcl XL Protein;Synonym:BclXL, Bcl-X(L), Bcl-XL.;Description:Bcl-XL Protein produced in E.coli is a single, non-glycosylated polypeptide chain containing amino acids 1-210. The Bcl-XL is purified by proprietary chromatographic techniques.;Source:E.coli;Purity:Greater than 95.0% as determined by SDS-PAGE.;Bioactivity:Please contact us for detailed information;Formulation:The protein contains 10mM Tris-HCl pH-8, 1mM EDTA and 250mM NaCl.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:Suspend BclXL in 1000µl sterile 18M-cm H2O, over night at 4°C. Dilute 10 fold into selected buffer system.Bcl-XL has tendency to form intramolecular disulfide bond, 5mM DTT is recommended in assay buffer. When running SDS-PAGE gel, 10mM DTT is recommended;Storage:Lyophilized Bcl-XL although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Bcl-XL should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.;
Bcl-XL Protein produced in E.coli is a single, non-glycosylated polypeptide chain containing amino acids 1-210. The Bcl-XL is purified by proprietary chromatographic techniques.
Source:
E.coli
Purity:
Greater than 95.0% as determined by SDS-PAGE.
Bioactivity:
Please contact us for detailed information
Formulation:
The protein contains 10mM Tris-HCl pH-8, 1mM EDTA and 250mM NaCl.
Stability:
Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃
Reconstitution:
Suspend BclXL in 1000µl sterile 18M-cm H2O, over night at 4°C. Dilute 10 fold into selected buffer system.Bcl-XL has tendency to form intramolecular disulfide bond, 5mM DTT is recommended in assay buffer. When running SDS-PAGE gel, 10mM DTT is recommended
Storage:
Lyophilized Bcl-XL although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Bcl-XL should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.