Cat#:RP-2054H;Product Name:Recombinant Human APOE3 Protein;Synonym:Apolipoprotein E, LDLCQ5, APO-E, LPG, AD2, Alzheimer Disease 2 (APOE*E4-Associated, Late Onset), Apolipoprotein E3, APOE.;Description:APOE3 Protein (19-317) produced in E.coli is a single, non-glycosylated, polypeptide chain containing 306 amino acids and having a molecular mass of 35kDa. The APOE is fused to a 7 amino acid His tag [M-HHHHHH] at N-terminus and purified by proprietary chromatographic techniques.;Source:E.coli;AA Sequence:MHHHHHHKVE QAVETEPEPE LRQQTEWQSG QRWELALGRF WDYLRWVQTL SEQVQEELLS SQVTQELRAL MDETMKELKA YKSELEEQLT PVAEETRARL SKELQAAQAR LGADMEDVCG RLVQYRGEVQ AMLGQSTEEL RVRLASHLRK LRKRLLRDAD DLQKRLAVYQ AGAREGAERG LSAIRERLGP LVEQGRVRAA TVGSLAGQPL QERAQAWGER LRARMEEMGS RT;Purity:Greater than 95% as determined by SDS-PAGE.;Bioactivity:Immobilized rHuApoE3 binds to rMuVLDLR with EC50 less than 0.4µg/ml.;Formulation:Lyophilized from a 0.2µm filtered solution in PBS.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized APOE in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized APOE3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution APOE should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.;