Cat#:RP-2260H;Product Name:Recombinant Human ALK-3 / BMPR1A Protein;Synonym:BMPR-1A, BMP-R1A, BMPR1A, BMR1A, CD292, CD-292, Serine/threonine-protein kinase receptor R5, SKR5, ALK-3, ACVRLK3, EC 2.7.11.30, CD292 antigen.;Description:Recombinant human ALK-3 / BMPR1A Protein extracellular domain produced in baculovirus is a monomeric, glycosylated, Polypeptide chain fused with 6xHis tag at C-terminus and having a molecular mass of 23 kDa. The recombinant human BMR1A protein is purified by our unique chromatographic techniques.;Source:Insect Cells.;Purity:Greater than 90.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.;Bioactivity:Measured by its ability to inhibit recombinant human BMP-2 induced alkaline phosphatase production by C2C12 myogenic cells. The ED50 for this effect is typically 1-3 µg/ml in the presence of 500 ng/ml of recombinant human BMP-2 corresponding to a Specific Activity of 2,000 units/mg.;Formulation:Recombinant human ALK-3 protein was lyophilized from a concentrated (1mg/ml) sterile solution containing 1X PBS.;Stability:Recombinant human ALK-3 Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized recoombinant human ALK-3 in sterile PBS not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized recombinant human ALK-3 protein although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution recombinant human BMPR1A protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.;References:Zielins ER, et al. Enrichment of Adipose-Derived Stromal Cells for BMPR1A Facilitates Enhanced Adipogenesis.Tissue Eng Part A, 2016 Feb. PMID 26585335;