Cat#:RP-1893H;Product Name:Recombinant Human Actin Protein;Synonym:Actin;Description:Ultra pure Actin consists in the alpha-skeletal muscle isoform and is purified from rabbit striated muscle. The purification method used (according to Spudich & Watts) results in a highly purified protein having a Molecular mass of 43,000 dalton.;Source:Rabbit Muscle.;Purity:Greater than 98.0% as determined by SDS-PAGE.;Bioactivity:Please contact us for detailed information;Formulation:The protein was lyophilized from a 1mg/ml solution containing 10mM Tris/HCl buffer pH 8.0, 0.2mM CaCl2, 0.2mM ATP, 1mM DTT and 0.5% (w/v) SDS.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized Actin in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized Actin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Actin should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.;
Ultra pure Actin consists in the alpha-skeletal muscle isoform and is purified from rabbit striated muscle. The purification method used (according to Spudich & Watts) results in a highly purified protein having a Molecular mass of 43,000 dalton.
Source:
Rabbit Muscle.
Purity:
Greater than 98.0% as determined by SDS-PAGE.
Bioactivity:
Please contact us for detailed information
Formulation:
The protein was lyophilized from a 1mg/ml solution containing 10mM Tris/HCl buffer pH 8.0, 0.2mM CaCl2, 0.2mM ATP, 1mM DTT and 0.5% (w/v) SDS.
Stability:
Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃
Reconstitution:
It is recommended to reconstitute the lyophilized Actin in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage:
Lyophilized Actin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Actin should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.