Cat#: | RPH-NP206 |
Product Name: | Recombinant Human Interleukin-10 / IL10 Protein(His Tag) |
Synonym: | Interleukin-10, IL-10, Cytokine Synthesis Inhibitory Factor, CSIF, IL10 |
Description: | Recombinant Human Interleukin-10 Protein is produced in E.coli and the target gene encoding Ser19-Asn178 is expressed with a His tag at the N-terminus. |
Source: | E.coli |
AA Sequence: | MGSSHHHHHHSSGLVPRGSHMSPGQGTQSENSCTHFPGNLPNMLRDLRDAFSRVKTFFQMKDQLD NLLLKESLLEDFKGYLGCQALSEMIQFYLEEVMPQAENQDPDIKAHVNSLGENLKTLRLRLRRCH RFLPCENKSKAVEQVKNAFNKLQEKGIYKAMSEFDIFINYIEAYMTMKIRN |
Purity: | Greater than 95% as determined by reducing SDS-PAGE. |
Endotoxin: | < 0.1 ng/µg (1 IEU/µg) as determined by LAL test. |
Bioactivity: | ED50 is less than 2 ng/ml. Specific Activity of 1.5 x 10^6 IU/mg. |
Formulation: | Recombinant Human Interleukin-10/IL10 Protein (His Tag)was lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Stability: | Recombinant Human Interleukin-10/IL10 Protein (His Tag)is stable for up to 1 year from date of receipt at -70℃. |
Reconstitution: | Please centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in ddH2O.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Usage: | For Lab Research Use Only |
Storage: | Lyophilized recombinant human IL10 Protein should be stored below -20°C, though stable at room temperature for 3 weeks. Reconstituted recombinant human IL10 protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted recombinant human IL10 protein samples are stable below -20°C for 3 months. |