Cat#:RPH-NP168;Product Name:Recombinant Human GM-CSF / CSF2 Protein;Synonym:Granulocyte-Macrophage Colony-Stimulating Factor, GM-CSF, Colony-Stimulating Factor, CSF, Molgramostin, Sargramostim, CSF2, GMCSF;Description:Recombinant Human Granulocyte-Macrophage Colony-Stimulating Factor Protein is produced in E.coli and the target gene encoding Ala18-Glu144 is expressed.;Source:E. coli;AA Sequence:MAPARSPSPSTQPWEHVNAIQEARRLLNLSRDTAAEMNETVEVISEMFDLQEPTCLQTRLELYKQ GLRGSLTKLKGPLTMMASHYKQHCPPTPETSCATQIITFESFKENLKDFLLVIPFDCWEPVQE;Purity:Greater than 95% as determined by reducing SDS-PAGE.;Endotoxin:< 0.1 ng/µg (1 IEU/µg) as determined by LAL test.;Bioactivity:ED50 is less than 0.1 ng/ml. Specific Activity of 1.0 x 10^7 IU/ mg. Measured by the dose-dependent stimulation of human TF-1 cell proliferation.;Formulation:Recombinant Human GM-CSF/CSF2 Protein was lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 5% Mannitol, pH 7.2.;Stability:Recombinant Human GM-CSF/CSF2 Protein is stable for up to 1 year from date of receipt at -70℃.;Reconstitution:Please centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in ddH2O.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.;Storage:Lyophilized recombinant human GM-CSF protein should be stored below -20°C, though stable at room temperature for 3 weeks. Reconstituted recombinant human GM-CSF protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted recombinant human GM-CSF protein samples are stable below -20°C for 3 months.;Usage:For Lab Research Use Only;