Cat#: | RP-RANKL-01H |
Product Name: | Recombinant Human RANKL / OPGL / TNFSF11 / CD254 Protein |
Synonym: | RANKL, OPGL,TNFSF11,CD254,hRANKL2 |
Description: | Recombinant human RANKL protein was produced in Human cells. A DNA sequence encoding the human RANKL/TNFSF11 (O14788-2) (Gly 63-Asp 244) was expressed with the Fc region of rabbit IgG at the N-terminus. The RANKL human recombinant protein was purified by our unique chromatographic techniques. |
Source: | Human Cells |
Predicted N Terminal: | Ser |
Molecular Characterization: | The recombinant human RANKL protein comprises 431 amino acids and has a predicted molecular mass of 47.9 kDa. The apparent molecular mass of the protein is approximately 56 kDa in SDS-PAGE under reducing conditions due to glycosylation. |
Purity: | Greater than 85 % as determined by SDS-PAGE |
Endotoxin: | < 1.0 EU per μg of the recombinant human rankl protein as determined by the LAL method |
Bioactivity: | The bioactivity of recombinant human RANKL protein was determined by measuring the ability of hRANKL to induce TRAP activity in Raw 264.7 cells. The ED50 for this effect is 1.5-7.5ng/mL. |
Formulation: | Recombinant human RANKL protein was lyophilized from sterile PBS, pH 7.4. |
Stability: | Recombinant human RANKL Proteins are stable for up to 1 year from date of receipt at -70℃ |
Storage: | Store recombinant human RANKL protein under sterile conditions at -20℃ to -80℃. It is recommended that the human rankl protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
References: | Leibbrandt A, et al. (2008) RANK/RANKL: regulators of immune responses and bone physiology. Ann N Y Acad Sci. 1143: 123-50. |