Cat#:RP-0694H;Product Name:Recombinant Human Flavokinase / RFK Protein (His Tag);Synonym:RFK; riboflavin kinase; RIFK;Background:Gene ID: 55312. RFK,the full name is riboflavin kinase. Riboflavin kinase (RFK; EC 2.7.1.26) is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis (Karthikeyan et al., 2003 [PubMed 12623014]).[supplied by OMIM, Nov 2009];Description:A DNA sequence encoding the mature form of human RFK (Q969G6) was expressed in E. coli. The human RFK protein was fused with a His tag at the N-terminus.;Source:E. coli;Predicted N Terminal:His;AA Sequence:Met1-His155;Molecular Characterization:The recombinant human RFK protein consists of 170 amino acids and predicts a molecular mass of 19.5 KDa. It migrates as an approximately 20 KDa band in SDS-PAGE under reducing conditions.;Purity:Greater than 90 % as determined by SDS-PAGE;Endotoxin:< 1.0 EU per ug of the human AACS protein as determined by the LAL method;Formulation:Liquid Solution;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Host Species:Human;Storage:Store the recombinant human RFK protein at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.;Usage:For Lab Research Use Only;Supplied in:The recombinant human RFK protein was supplied in 20mM Tris, 10% glycerol, pH 8.0;References:Crystal structure of human riboflavin kinase reveals a beta barrel fold and a novel active site arch. Karthikeyan S, et al. Structure, 2003 Mar. PMID 12623014;