Cat#: | RP-6201H |
Product Name: | Recombinant Human TPO Protein |
Synonym: | Megakaryocyte colony-stimulating factor, Myeloproliferative leukemia virus oncogene ligand, C-mpl ligand, ML, Megakaryocyte growth and development factor, MGDF, TPO, MKCSF, MPLLG, MGC163194, THPO. |
Description: | Thrombopoietin Protein produced in E.coli is a single, non-glycosylated soluble polypeptide chain containing 175 amino acids and having a molecular mass of 18.8kDa which comprises the receptor binding domain of the Mpl-ligand protein. The TPO is purified by proprietary chromatographic techniques. |
Source: | E.coli |
AA Sequence: | MSPAPPACDLR VLSKLLRDSH VLHSRLSQCP EVHPLPTPVL LPAVDFSLGE WKTQMEETKA QDILGAVTLL LEGVMAARGQ LGPTCLSSLL GQLSGQVRLL LGALQSLLGT QLPPQGRTTA HKDPNAIFLS FQHLLRGKVR FLMLVGGSTL CVRRAPPTTA VPSRTSLVLT LNEL. |
Purity: | Greater than 90.0% as determined by SDS-PAGE. |
Bioactivity: | The ED50, as determined by the dose-dependent proliferation of MO7e cells (with SCF), is 1.95 ng/ml, corresponding to a specific activity of 5x105 units/mg. |
Formulation: | Lyophilized from a sterile filtered solution containing 10mM sodium phosphate, pH 7.5. |
Stability: | Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃ |
Reconstitution: | It is recommended to reconstitute the lyophilized Thrombopoietin in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Storage: | Lyophilized Thrombopoietin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TPO Human should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |