Cat#: | RP-4456H |
Product Name: | Recombinant Maltose Binding Protein / MBP Protein |
Synonym: | MBP,Maltose Binding Protein |
Gene Introduction: | Maltose-binding protein (MBP) is a part of the maltose/maltodextrin system of Escherichia coli, which is responsible for the uptake and efficient catabolism of maltodextrins. It is a complex regulatory and transport system involving many proteins and protein complexes. MBP has an approximate molecular mass of 42.5 kilodaltons. |
Description: | Recombinant Maltose Binding Protein expressed in E.coli is a single, non-glycosylated polypeptide chain containing 387aa (27 to 392) with additional 20aa linker and having a molecular mass of 42kDa. |
Source: | E.coli |
AA Sequence: | MKIEEGKLVI WINGDKGYNG LAEVGKKFEK DTGIKVTVEH PDKLEEKFPQ VAATGDGPDI IFWAHDRFGG YAQSGLLAEI TPDKAFQDKL YPFTWDAVRY NGKLIAYPIAVEALSLIYNK DLLPNPPKTW EEIPALDKEL KAKGKSALMF NLQEPYFTWP LIAADGGYAF KYENGKYDIK DVGVDNAGAK AGLTFLVDLI KNKHMNADTD YSIAEAAFNK GETAMTINGP WAW |
Molecular Characterization: | 42kDa |
Purity: | Greater than 95.0% as determined by SDS-PAGE. |
Formulation: | The Maltose Binding Protein solution (1 mg/ml) contains 20mM Tris-HCl pH-8. |
Stability: | Recombinant MBP Proteins are stable for up to 1 year from date of receipt at -70℃ |
Storage: | Store Maltose-binding protein at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles. |
References: | Duplay, P; Bedouelle, H; Fowler, A; Zabin, I; Saurin, W; Hofnung, M (1984). "Sequences of the malE gene and of its product, the maltose-binding protein of Escherichia coli K12". J Biol Chem. 259 (16): 10606–10613. |