Cat#: | RP-4046H |
Product Name: | Recombinant Human IL2 Protein, Yeast |
Synonym: | Interleukin-2, T-cell growth factor (TCGF), Aldesleukin, Lymphokine, IL-2. |
Description: | Recombinant human Interleukin-2 / IL2 Protein produced in yeast is a single, glycosylated polypeptide chain containing 134 amino acids and having a molecular mass of 14 kDa. The recombinant human IL-2 protein is purified by our proprietary chromatographic techniques. |
Source: | Pichia pastoris. |
AA Sequence: | A P T S S S T K K T Q L Q L E H L L L D L Q M I L N G I N N Y K N P K L T R M L T F K F Y M P K K A T E L K H L Q C L E E E L K P L E E V L N L A Q S K N F H L R P R D L I S N I N V I V L E L K G S E T T F M C E Y A D E T A T I V E F L N R W I T F C Q S I |
Purity: | Greater than 98% as determined by SDS-PAGE. |
Bioactivity: | The biological activity of recombinant human IL2 protein is determined by the dose dependent proliferation of mouse CTLL–2 cells. Optimal concentration for individual application should be determined by a dose response assay. ED50 range = 0.08–0.5ng/ml |
Formulation: | The human IL2 protein was lyophilized from a 0.2µm filtered solution in 20mM sodium phosphate buffer pH 7.0 in absence of any carrier protein. |
Stability: | Recombinant human IL2 Proteins are stable for up to 1 year from date of receipt at -70℃ |
Reconstitution: | It is recommended to reconstitute the lyophilized Interleukin-2 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Storage: | Lyophilized recombinant human Interleukin-2 / IL2 protein although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution recombinant Interleukin-2 / IL2 protein should be stored at 4C between 2-7 days and for future use below -18C. Please avoid freeze-thaw cycles. |