Cat#: | RP-1681H |
Product Name: | Recombinant Human ERN1 / IRE1 Protein (aa 465-977, His & GST Tag) |
Synonym: | hIRE1p,IRE1,IRE1a,IRE1P |
Description: | A DNA sequence encoding the human ERN1 isoform 1 (O75460-1) cytoplasmic domain (Pro 465-Leu 977) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus. |
Source: | Baculovirus-Insect Cells |
Predicted N Terminal: | Met |
Molecular Characterization: | The recombinant human ERN1/GST chimera consists of 750 amino acids and has a calculated molecular mass of 86 kDa. It migrates as an approximately 90 kDa band in SDS-PAGE under reducing conditions. |
Purity: | Greater than 80 % as determined by SDS-PAGE |
Endotoxin: | < 1.0 EU per ug of the protein as determined by the LAL method |
Bioactivity: | Please contact us for detailed information |
Formulation: | Supplied as sterile 20mM Tris, 500mM NaCl, 10% gly, pH 7.4 |
Stability: | Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃ |
Reconstitution: | Please see related COA for specific instructions. |
Storage: | Avoid repeated freeze-thaw cycles. In lyophilized state for 1 year (4-8℃); Upon reconstitution under sterile conditions for 2 weeks (4-8℃) or 3 months (-20℃ to -70℃). |