Cat#:AB-001EPH;Product Name:Recombinant Human EPO Protein(His Tag);Synonym:EPO, EP, Erythropoietin, Erythropoetin, Erthropoyetin, Hematopoietin, Hemopoietin, rhEPO, MVCD3;Background:This gene encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The encoded protein is mainly synthesized in the kidney, secreted into the blood plasma, and binds to the erythropoietin receptor to promote red blood cell production, or erythropoiesis, in the bone marrow. Expression of this gene is upregulated under hypoxic conditions, in turn leading to increased erythropoiesis and enhanced oxygen-carrying capacity of the blood. Expression of this gene has also been observed in brain and in the eye, and elevated expression levels have been observed in diabetic retinopathy and ocular hypertension. Recombinant forms of the encoded protein exhibit neuroprotective activity against a variety of potential brain injuries, as well as antiapoptotic functions in several tissue types, and have been used in the treatment of anemia and to enhance the efficacy of cancer therapies. [provided by RefSeq, Aug 2017];Description:Recombinant Human Erythropoietin / EPO is produced by our Mammalian expression system and the target gene encoding Ala28-Arg193 is expressed with a 6His tag at the C-terminus;Source:Human Cells;Molecular Characterization:19.2kDa. AP Mol Mass 30-40kDa, reducing conditions.;Purity:Greater than 95% by SDS-PAGE;Endotoxin:< 0.1 ng/µg (1 IEU/µg) as determined by LAL test.;Bioactivity:Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is less than 5 ng/ml;Formulation:The recombinant human EPO protein was lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.;Reconstitution:Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water. ;Host Species:Human;Storage:Lyophilized epo protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted recombinant epo protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months;Usage:For Lab Resarch Use Only!;