Cat#:RP-10645H;Product Name:Recombinant Human IDE Protein(His Tag);Synonym:IDE, insulin degrading enzyme,INSULYSIN;Background:This gene encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described but have not been experimentally verified.[provided by RefSeq, Sep 2009];Description:Recombinant Human IDE Protein expressed in E.Coli is a single, non-glycosylated, polypeptide chain containing 1,026aa including a 7 aa His tag at C-terminus. The Human insulin degrading enzyme is purified using our unique purification techniques.;Source:E. coli;AA Sequence:Met1-Leu1019;Molecular Characterization:The total calculated molecular mass is 119kDa.;Purity:Greater than 95% by SDS-PAGE;Formulation:Filtered colorless solution.Human IDE filtered (0.4µm) solution at a concentration of 0.6mg/ml in 20mM Tris buffer, 50mM NaCl, pH 8.0 and 10% (w/v) glycerol.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Host Species:Human;Storage:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.;References:Toward Allosterically Increased Catalytic Activity of Insulin-Degrading Enzyme against Amyloid Peptides. Kurochkin IV, et al. Biochemistry, 2017 Jan 10. PMID 27982586;