Cat#:WT1-RP10H;Product Name:Recombinant Human WT1 Protein(Flag Tag);Synonym:GUD; AWT1; WAGR; WT33; NPHS4; WIT-2; WT1; Wilms tumor 1;Background:This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq, Mar 2015];Description:Recombinant human WT1 protein was expressed in Baculovirus-insect cell having a molecular weight of 55.7 kDa. The residue is 1-446, including exon 5 but without KTS. The recombinant WT1 protein is purified by affinity chromatography in combination with FPLC columns. Recombinant WT1 protein can be used for: 1) protein-protein interaction assay; 2) in vitro function studies including transcription, DNA or RNA binding assays; and 3) cell growth and proliferation assays.;Source:Baculovirus-insect cell;AA Sequence:1-446;Molecular Characterization:55.7 kDa.;Purity:Greater than 95% as determined by SDS-PAGE;Bioactivity:Active;Formulation:Liquid Solution;Stability:Recombinant human WT1 proteins are stable for up to 1 year from date of receipt at -70℃;Host Species:Human;Storage:Stored recombinant human WT1 protein at -70°C before use. Please avoid repeated freeze thaw cycles.;Usage:For Lab Research Use Only;Supplied in:1x dilution buffer A: 20 mM Tris-Cl (pH 8.0), 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA;References:The CUG-translated WT1, not AUG-WT1, is an oncogene. Lee KY, et al. Carcinogenesis, 2017 Dec 7. PMID 29040381;