Cat#:ADV-2967EK;Product Name:Rat Erythropoietin, EPO ELISA Kit;Size:96T, 5×96T, 10×96T;Sample:Serum, Plasma, Tissue Homogenates;Synonym:EpoErythropoietin ELISA Kit;Description:This Rat EPO ELISA Kit was designed for the quantitative measurement of Rat EPO protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL .;Species Reactivity:Rat;Usage:For Research Use Only;Detection Principle:Sandwich ELISA;Contents of Kit:A micro ELISA plate --The 96-well plate has been pre-coated with an anti-rat EPO antibody. This dismountable microplate can be divided into 12 x 8 strip plates. Two vials lyophilized standard --Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve. One vial Biotin-labeled EPO antibody (100 x concentrate) (120 μl/bottle) --Act as the detection antibody. One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic. One vial Biotin-antibody Diluent (15 ml/bottle) --Dilute the high concentration Biotin-antibody to an appropriate working solution. One vial HRP-avidin Diluent (15 ml/bottle) --Dilute the high concentration HRP-avidin solution to an appropriate solution. One vial Sample Diluent (50 ml/bottle)--Dilute the sample to an appropriate concentration. One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances. One vial TMB Substrate (10 ml/bottle) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue. One vial Stop Solution (10 ml/bottle) --Stop the color reaction. The solution color immediately turns from blue to yellow. Four Adhesive Strips (For 96 wells) --Cover the microplate when incubation. An instruction manual;Detection Range:0.156 ng/mL-10 ng/mL;Sensitivity:0.039 ng/mL;