Cat#:EIA-416C;Product Name:Human SERPINA1 ELISA Kit;Size:96T;Sample:serum, plasma, milk, and colostrum;Intended use:RUO;Synonym:SERPINA1; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1; PI; A1A; AAT; PI1; A1AT; MGC9222; PRO2275; MGC23330; alpha1AT; alpha-1-antitrypsin; serpin A1; OTTHUMP00000197150; OTTHUMP00000197151; OTTHUMP00000197152; OTTHUMP00000197153; OTTHUMP00000197154; OTTHUMP00000245864; alpha-1-antiproteinase; alpha-1-antitrypsin null; alpha-1 protease inhibitor; protease inhibitor 1 (anti-elastase), alpha-1-antitrypsin; serine (or cysteine) proteinase inhibitor, clade A, member 1;Background:Human alpha-1 antitrypsin (A1AT) is a 52-kDa glycoprotein that belongs to a class of proteins called serpins, or serine protease inhibitors. These proteins inactivate proteases by binding to them covalently. This is an extremely important role since physiological conditions that lead to increased or potentially uncontrolled activities of the proteases can eventually lead to tissue breakdown. For example, a hereditary disease called alpha-1 antitrypsin deficiency causes degradation of lung tissues that result in pulmonary emphysema.The concentrations of A1AT in normal serum or plasma, colostrum, and mature milk are 1.5-3.5 mg/ml, 0.1-0.5 mg/ml, and 3-10 µg/ml, respectively. However, the levels can change significantly upon infection, inflammation, or certain diseased states. Although the use of recombinant A1AT has not yet been approved for human applications, concentrates from human plasma have been approved for therapeutic use. Therefore, an accurate quantitation of A1AT in plasma or serum is important for research diagnostics purposes, as well as for monitoring the efficacy of treatment regimens.;Species Reactivity:Human;Application:quantitate;Storage:Unopened Kit: Store at 2 - 8°C. Do not use past kit expiration date. Opened/Reconstituted Reagents: TMB Solution A; TMB Solution B; TMB Stop Solution; Wash Buffer; HRP-conjugate antibody The above mentioned reagents should be stored for up to 1 month at 2 - 8°C. Microplate Wells: Return unused wells to the foil pouch containing the desiccant pack, reseal along entire edge of zip-seal. May be stored for up to 1 month at 2 - 8°C.;Usage:For Lab Research Use Only;Detection Principle:Sandwich ELISA;
Human alpha-1 antitrypsin (A1AT) is a 52-kDa glycoprotein that belongs to a class of proteins called serpins, or serine protease inhibitors. These proteins inactivate proteases by binding to them covalently. This is an extremely important role since physiological conditions that lead to increased or potentially uncontrolled activities of the proteases can eventually lead to tissue breakdown. For example, a hereditary disease called alpha-1 antitrypsin deficiency causes degradation of lung tissues that result in pulmonary emphysema.The concentrations of A1AT in normal serum or plasma, colostrum, and mature milk are 1.5-3.5 mg/ml, 0.1-0.5 mg/ml, and 3-10 µg/ml, respectively. However, the levels can change significantly upon infection, inflammation, or certain diseased states. Although the use of recombinant A1AT has not yet been approved for human applications, concentrates from human plasma have been approved for therapeutic use. Therefore, an accurate quantitation of A1AT in plasma or serum is important for research diagnostics purposes, as well as for monitoring the efficacy of treatment regimens.
Species Reactivity:
Human
Application:
quantitate
Usage:
For Lab Research Use Only
Detection Principle:
Sandwich ELISA
Storage:
Unopened Kit: Store at 2 - 8°C. Do not use past kit expiration date. Opened/Reconstituted Reagents: TMB Solution A; TMB Solution B; TMB Stop Solution; Wash Buffer; HRP-conjugate antibody The above mentioned reagents should be stored for up to 1 month at 2 - 8°C. Microplate Wells: Return unused wells to the foil pouch containing the desiccant pack, reseal along entire edge of zip-seal. May be stored for up to 1 month at 2 - 8°C.
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