Cat#:ADV-0219EK;Product Name:Human Osteopontin, OPN ELISA Kit;Size:96T, 5×96T, 10×96T;Sample:Serum, Plasma, Urine, Tissue Homogenates;Synonym:BNSP ELISA Kit; Bone sialoprotein 1 ELISA Kit; Bone sialoprotein I ELISA Kit; BSP I ELISA Kit; BSPI ELISA Kit; Early T lymphocyte activation 1 ELISA Kit; ETA 1 ELISA Kit; ETA1 ELISA Kit; MGC110940 ELISA Kit; Nephropontin ELISA Kit; OPN ELISA Kit; Osteopontin ELISA Kit; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein ELISA Kit; OSTP_HUMAN ELISA Kit; PSEC0156 ELISA Kit; secreted phosphoprotein 1 (osteopontin bone sialoprotein I early T lymphocyte activation 1) ELISA Kit; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1) ELISA Kit; Secreted phosphoprotein 1 ELISA Kit; SPP 1 ELISA Kit; SPP-1 ELISA Kit; SPP1 ELISA Kit; SPP1/CALPHA1 fusion ELISA Kit; Urinary stone protein ELISA Kit; Uropontin ELISA Kit;Description:The Human Osteopontin (OPN) ELISA Kit is a powerful tool for researchers in the field of cancer. This quantitative assay measures levels of OPN, a protein involved in the regulation of bone remodeling and implicated in various diseases including cancer（tumor），bone metabolism, immunity, growth and development. With a detection range of 0.78 ng/mL-50 ng/mL and a sensitivity of 0.195 ng/mL, this ELISA kit can detect low levels of OPN with high accuracy. It can be used with a variety of sample types, including serum, plasma, urine, and tissue homogenates, making it a versatile tool for research. The assay principle is based on the sandwich method, where a monoclonal antibody specific to human OPN is pre-coated onto a microplate. The sample is added, and any OPN present in the sample binds to the antibody. A biotinylated polyclonal antibody specific to human OPN is then added, which binds to the OPN-antibody complex. Finally, streptavidin conjugated to horseradish peroxidase (HRP) is added, which binds to the biotinylated antibody. The HRP produces a colorimetric reaction in the presence of a substrate, which can be measured at a wavelength of 450 nm. The assay can be completed in as little as 1-5 hours and requires a sample volume of only 50-100ul, minimizing the amount of sample required for analysis. The kit includes all necessary components and can be used with standard laboratory equipment. With 12 citations, this kit has been validated by the scientific community and is a trusted tool for cancer research. So, whether you are studying the role of OPN in cancer progression or investigating potential therapeutic targets, this Human Osteopontin (OPN) ELISA Kit is an essential addition to your research arsenal.;Species Reactivity:Human;Usage:For Research Use Only;Detection Principle:Sandwich ELISA;Contents of Kit:A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human OPN antibody. This dismountable microplate can be divided into 12 x 8 strip plates. Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve. One vial Biotin-labeled OPN antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody. One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic. One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody. One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution. One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration. One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances. One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue. One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow. Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation. An instruction manual;Detection Range:0.78 ng/mL-50 ng/mL;Sensitivity:0.195 ng/mL;