Cat#:EIA-438C;Product Name:Human NPPA ELISA Kit;Size:96T;Sample:EDTA PLASMA, HEPARIN PLASMA, SERUM, URINE OR CELL CULTURE SUPERNATANTS;Intended use:N/A;Synonym:NPPA; natriuretic peptide A; ANF; ANP; PND; ATFB6; CDD-ANF; atriopeptin; cardionatrin; OTTHUMP00000002504; prepronatriodilatin; natriuretic peptide precursor; A; atrial natriuretic factor; Prepronatriodilatin; Atrial natriuretic peptide; ATFB6;Background:Atrial natriuretic peptide is synthesized in atrial myocytes and is stored in secretory granules as a 126 amino acid prohormone. The most important stimulus for the release of the hormone into circulation is stretch of the myocyte fibres. On release the prohormone is split into equimolar amounts of the highly biologically active proANP (99-126), also known as α-ANP, and the N-terminal part proANP (1-98). α-ANP is rapidly cleared from the circulation with a half-life of 3-4 minutes. proANP (1-98) has a much longer half-life (60-120 min) which leads to significantly higher concentrations in blood compared to α-ANP. Thus, circulating levels of proANP (1-98) are less sensitive to the pulsatile secretion of ANP and may better reflect chronic levels of ANP secretion than the rapidly fluctuating levels of α-ANP. proANP is discussed as valuable marker for e.g. sepsis, or risk stratification in heart failure.;Species Reactivity:Human;Application:QUANTITATIVE;Storage:Unopened Kit: Store at 2 - 8°C. Do not use past kit expiration date. Opened/Reconstituted Reagents: TMB Solution A; TMB Solution B; TMB Stop Solution; Wash Buffer; HRP-conjugate antibody The above mentioned reagents should be stored for up to 1 month at 2 - 8°C. Microplate Wells: Return unused wells to the foil pouch containing the desiccant pack, reseal along entire edge of zip-seal. May be stored for up to 1 month at 2 - 8°C.;Usage:For Lab Research Use Only;Detection Principle:Sandwich ELISA;