Cat#:ADV-2357EK;Product Name:Human Mucin 2(MUC2) ELISA Kit;Size:96T, 5×96T, 10×96T;Sample:Serum, Plasma, Cell Culture Supernates;Synonym:Intestinal mucin 2 ELISA Kit; Intestinal mucin-2 ELISA Kit; MLP ELISA Kit; MUC 2 ELISA Kit; MUC-2 ELISA Kit; Muc2 ELISA Kit; MUC2_HUMAN ELISA Kit; Mucin 2 ELISA Kit; Mucin 2 intestinal ELISA Kit; Mucin 2 intestinal/tracheal ELISA Kit; Mucin 2 oligomeric mucus/gel forming ELISA Kit; Mucin 2 precursor ELISA Kit; Mucin 2 tracheal ELISA Kit; Mucin like protein ELISA Kit; Mucin-2 ELISA Kit; Mucin2 ELISA Kit; SMUC ELISA Kit;Description:The human Mucin 2 (MUC2) ELISA kit is essential for quantifying MUC2 levels in human serum, plasma, and cell culture supernatants. It detects concentrations from 3.12 ng/mL to 200 ng/mL, with a sensitivity of 0.78 ng/mL. Utilizing a sandwich method, this kit ensures accurate and reliable measurements. It takes about 1-5 hours to accomplish the detection and needs only 50-100 µL of the sample. Detection occurs at a wavelength of 450 nm. The kit's effectiveness is well-documented in various experimental studies, demonstrating its reliability for research purposes. The human MUC2 is a critical mucin protein that plays a significant role in forming a protective mucous layer in the intestines [1]. MUC2 regulates the expression and antimicrobial activity of β-defensin 2 in the colon, highlighting its role in the immune response and defense mechanisms in the gastrointestinal tract [2]. Furthermore, MUC2 contributes to synthesizing the antimicrobial peptide cathelicidin in response to colitis, emphasizing its involvement in the innate defenses of the colon against pathogens [3]. References:[1] Z. Jiang, T. Applegate, & A. Lossie, "Cloning, annotation and developmental expression of the chicken intestinal muc2 gene", Plos One, vol. 8, no. 1, p. e53781, 2013. https://doi.org/10.1371/journal.pone.0053781[2] E. Cobo, F. Moreau, & K. Chadee, "Colonic muc2 mucin regulates the expression and antimicrobial activity of β-defensin 2", Mucosal Immunology, vol. 8, no. 6, p. 1360-1372, 2015. https://doi.org/10.1038/mi.2015.27[3] E. Cobo, V. Kissoon‐Singh, R. Holani, & K. Chadee, "Muc2 mucin and butyrate contribute to the synthesis of the antimicrobial peptide cathelicidin in response to entamoeba histolytica- and dextran sodium sulfate-induced colitis", Infection and Immunity, vol. 85, no. 3, 2017. https://doi.org/10.1128/iai.00905-16;Species Reactivity:Human;Usage:For Research Use Only;Detection Principle:Sandwich ELISA;Contents of Kit:A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human MUC2 antibody. This dismountable microplate can be divided into 12 x 8 strip plates. Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve. One vial Biotin-labeled MUC2 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody. One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic. One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody. One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution. One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration. One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances. One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue. One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow. Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation. An instruction manual;Detection Range:3.12 ng/mL-200 ng/mL;Sensitivity:0.78 ng/mL;