Cat#:EIA-453C;Product Name:Human LTF ELISA Kit;Size:96T;Sample:plasma, urine, milk and cerebrospinal fluid;Intended use:RUO;Synonym:LTF; lactotransferrin; LF; HLF2; GIG12; talalactoferrin; OTTHUMP00000209779; OTTHUMP00000209780; OTTHUMP00000209781; neutrophil lactoferrin; growth-inhibiting protein 12; EC 3.4.21; Lactoferrin;Background:Lactoferrin, also known as lactotransferrin, is an iron-binding glycoprotein that belongs to the transferrin family of proteins. This globular protein has a molecular mass of 80 kDa and contains four identical domains. Each pair of domains surrounds an iron (Fe) atom.First isolated in milk, lactoferrin has been also found in many mucosal secretions, such as tears and saliva. Lactoferrin is also present in secondary granules of polymorphonuclear cells and is also secreted by some acinar cells. Human colostrum has the highest lactoferrin concentration, followed by human milk, and then cow milk. Lactoferrin is generally purified from milk or produced recombinantly.Lactoferrin is a multifunctional protein that is part of the innate defense against infection and inflammation. Its long known antimicrobial activity is at least partly attributed to the ability of the apoprotein to sequester two atoms of iron, an essential bacterial nutrient. Proteolysis of lactoferrin produces lactoferricin, which has an even enhanced antimicrobial activity. This is attributed to its ability to disrupt the membrane structure, which eventually results in cell lysis.Other lactoferrin functions include inhibition of binding of bacterial and viral particles into host cells, or with each other. It has also antifungal activity. Lactoferrin receptors have been found on brush-border cells, monocytes, macrophages, and activated lymphocytes. More recently, lactoferrin was found to inhibit dendritic cell-mediated HIV-1 transmission by blocking the interaction of gp120 to DC-SIGN, which is a critical HIV protein that never changes regardless of strain. These broad ranges of functions clearly reflect the biological importance of lactoferrin.;Species Reactivity:Human;Application:quantitate;Storage:Unopened Kit: Store at 2 - 8°C. Do not use past kit expiration date. Opened/Reconstituted Reagents: TMB Solution A; TMB Solution B; TMB Stop Solution; Wash Buffer; HRP-conjugate antibody The above mentioned reagents should be stored for up to 1 month at 2 - 8°C. Microplate Wells: Return unused wells to the foil pouch containing the desiccant pack, reseal along entire edge of zip-seal. May be stored for up to 1 month at 2 - 8°C.;Usage:For Lab Research Use Only;Detection Principle:Sandwich ELISA;