Cat#:ADV-0623EK;Product Name:Human Leptin, LEP ELISA kit;Size:96T, 5×96T, 10×96T;Sample:Serum, Plasma, Tissue Homogenates;Synonym:FLJ94114 ELISA Kit; LEP ELISA Kit; LEP_HUMAN ELISA Kit; LEPD ELISA Kit; Leptin (murine obesity homolog) ELISA Kit; Leptin (obesity homolog, mouse) ELISA Kit; Leptin ELISA Kit; Leptin Murine Obesity Homolog ELISA Kit; Leptin Precursor Obesity Factor ELISA Kit; OB ELISA Kit; Obese protein ELISA Kit; Obese, mouse, homolog of ELISA Kit; Obesity ELISA Kit; Obesity factor ELISA Kit; Obesity homolog mouse ELISA Kit; Obesity Murine Homolog Leptin ELISA Kit; OBS ELISA Kit; OTTHUMP00000212285 ELISA Kit;Description:This Human LEP ELISA Kit was designed for the quantitative measurement of Human LEP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.060 ng/mL.;Species Reactivity:Human;Usage:For Research Use Only;Detection Principle:Sandwich ELISA;Contents of Kit:A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human LEP antibody. This dismountable microplate can be divided into 12 x 8 strip plates. Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve. One vial Biotin-labeled LEP antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody. One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic. One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody solution. One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution. One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration. One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances. One vial TMB Substrate (10 ml/bottle) --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue. One vial Stop Solution (10 ml/bottle) --- Stop the color reaction. The solution color immediately turns from blue to yellow. Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation. An instruction manual;Detection Range:0.156 ng/mL-10 ng/mL;Sensitivity:0.060 ng/mL;