Cat#:ADV-0017EK;Product Name:Human Homocysteine(HCY)ELISA Kit;Size:96T, 5×96T, 10×96T;Sample:Serum, Plasma, Urine, Tissue Homogenates;Description:This Human Hcy ELISA Kit was designed for the quantitative measurement of Human Hcy protein in serum, plasma, urine, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.78 nmol/mL-50 nmol/mL and the sensitivity is 0.195 nmol/mL .;Species Reactivity:Human;Usage:For Research Use Only;Detection Principle:Sandwich ELISA;Contents of Kit:A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human HCY antibody. This dismountable microplate can be divided into 12 x 8 strip plates. Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve. One vial Biotin-labeled HCY antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody. One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic. One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody. One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution. One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration. One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances. One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue. One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow. Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation. An instruction manual;Detection Range:0.78 nmol/mL-50 nmol/mL;Sensitivity:0.195 nmol/mL;