Cat#:ADV-1023EK;Product Name:Human Erythropoietin, EPO ELISA Kit;Size:96T, 5×96T, 10×96T;Synonym:EPOErythropoietin ELISA kit; Epoetin ELISA kit;Description:The human Erythropoietin (EPO) ELISA kit (CSB-E04538h) is designed for the quantitative measurement of human EPO protein in serum, plasma, or tissue homogenates. It quantitates human EPO with 1.95 mIU/ml sensitivity and shows excellent specificity for human EPO. It uses the bi-antibody sandwich enzyme immunoassay technique. This assay employs a biotin-conjugated EPO antibody that recognizes the analyte bound by the immobilized EPO antibody, forming an antibody-analyte-antibody complex. The immune complex is further detected by avidin-conjugated HRP. The TMB solution is added into the wells and turns blue and finally turns yellow after the addition of the stop solution. Solution color develops in proportion to the amount of EPO in the sample. The O.D. value is measured using a microplate reader at 450 nm and is used to determine the concentration of the human EPO in the sample. EPO is generated in the kidney in mammals and plays an essential role in bone marrow erythropoiesis. EPO is a major regulator of erythropoiesis by promoting the survival, proliferation, and differentiation of erythroid progenitor cells and modulating the number of erythrocytes in peripheral blood. Signaling pathways downstream of EPO/EPOR have been shown to influence numerous cellular functions in both normal and tumor cells, including proliferation, apoptosis, and drug resistance. EPO binds to its receptor EPOR, initiating signaling including PI3K and JAK-STAT pathways that promote growth, repress apoptosis, and induce the differentiation of erythroid progenitors to elevate red blood cell mass. Signaling pathways downstream of EPO-EPOR aix have been shown to affect multiple cellular functions in both normal and cancer cells, including proliferation, apoptosis, and drug resistance.;Species Reactivity:Human;Usage:For Research Use Only;Detection Principle:Sandwich ELISA;