Cat#:ADV-0274EK;Product Name:Human E-Selectin ELISA kit;Size:96T, 5×96T, 10×96T;Sample:Serum, Plasma, Tissue Homogenates;Synonym:SELE; ELAM1; E-selectin; CD62 antigen-like family member E; Endothelial leukocyte adhesion molecule 1; ELAM-1; Leukocyte-endothelial cell adhesion molecule 2; LECAM2; CD antigen CD62E;Description:The human SELE ELISA kit is a solid-phase immunoassay specially designed to quantitatively measure human SELE in serum, plasma, or homogenates. It is based on the Sandwich-ELISA mechanism. SELE in the sample is bound to the capture antibody immobilized on the 96-well strip plate and then sandwiched with the biotinylated SELE antibody. After the addition of HRP-avidin and TMB substrate, the solution in the wells turns blue. The color reaction is stopped by adding the stop solution into the wells, and the color changes from blue to yellow. The color intensity is positively proportional to the SELE bound in the initial step. The SELE concentration can be calculated according to the standard curve. This kit is tested with high sensitivity and strong specificity. SELE is a glycoprotein expressed on inflamed vascular endothelial cells in response to the inflammatory cytokines TNF-α and IL-1β. It mediates leukocyte rolling and vascular adhesion through interaction with carbohydrate ligands and thus is involved in the recruitment of neutrophils, monocytes, and T cells to inflammatory foci. Many studies have shown that endothelial cell SELE plays an important role in mediating cell-cell interactions between tumor cells and endothelial monolayers during tumor metastasis. SELE-mediated adhesion of circulating tumor cells to vascular endothelial cells (CTCs) initiates CTC extravasation at a distant site and is hence a critical step of the metastatic cascade.;Species Reactivity:Human;Usage:For Research Use Only;Detection Principle:Sandwich ELISA;Detection Range:0.312 ng/mL-20 ng/mL;Sensitivity:0.078 ng/mL;