Cat#:ADV-3319EK;Product Name:Human Complement 3, C3 ELISA Kit;Size:96T, 5×96T, 10×96T;Sample:Serum, Plasma, Cell Culture Supernates, Urine, Saliva;Synonym:ASP ELISA Kit; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1 ELISA Kit; C3 ELISA Kit; C3adesArg ELISA Kit; C3bc ELISA Kit; CO3_HUMAN ELISA Kit; Complement C3c alpha'' chain fragment 2 ELISA Kit;Description:This Human Complement 3 (C3) ELISA Kit was designed for the quantitative measurement of Human Complement 3 (C3) protein in serum, plasma, cell culture supernates, urine, saliva. It is a Sandwich ELISA kit, its detection range is 4.7 ng/mL-300 ng/mL and the sensitivity is 4.031 ng/mL.;Species Reactivity:Human;Usage:For Research Use Only;Detection Principle:Sandwich ELISA;Contents of Kit:A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human C3 antibody. Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve. One vial HRP-conjugated C3 antibody(100 x concentrate)(120 μl/bottle) --- Bind to the C3 in the samples or standards and react with the substrate to make the solution chromogenic. One vial Wash Buffer (25x concentrate) (20 ml/bottle) --- Wash away unbound or free substances. One TMBSubstrate (10 ml/bottle) ---Interact with HRP to elicit a chromogenic reaction. One vial Stop Solution (10ml/bottle) --- Stop the color reaction. The solution color immediately turns from blue to yellow. Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation. An instruction manual;Detection Range:4.7 ng/mL-300 ng/mL;Sensitivity:4.031 ng/mL;
This Human Complement 3 (C3) ELISA Kit was designed for the quantitative measurement of Human Complement 3 (C3) protein in serum, plasma, cell culture supernates, urine, saliva. It is a Sandwich ELISA kit, its detection range is 4.7 ng/mL-300 ng/mL and the sensitivity is 4.031 ng/mL.
Species Reactivity:
Human
Usage:
For Research Use Only
Detection Principle:
Sandwich ELISA
Contents of Kit:
A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human C3 antibody. Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve. One vial HRP-conjugated C3 antibody(100 x concentrate)(120 μl/bottle) --- Bind to the C3 in the samples or standards and react with the substrate to make the solution chromogenic. One vial Wash Buffer (25x concentrate) (20 ml/bottle) --- Wash away unbound or free substances. One TMBSubstrate (10 ml/bottle) ---Interact with HRP to elicit a chromogenic reaction. One vial Stop Solution (10ml/bottle) --- Stop the color reaction. The solution color immediately turns from blue to yellow. Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation. An instruction manual