Cat#:ADV-3288EK;Product Name:Human Calreticulin, CRT ELISA Kit;Size:96T, 5×96T, 10×96T;Sample:Serum, Plasma, Urine,Tissue Homogenates;Synonym:Autoantigen RO ELISA Kit; CALR ELISA Kit; CALR protein ELISA Kit; CALR_HUMAN ELISA Kit; Calregulin ELISA Kit; Calreticulin ELISA Kit; cC1qR ELISA Kit; CRP55 ELISA Kit; CRT ELISA Kit; CRTC ELISA Kit; Endoplasmic reticulum resident protein 60 ELISA Kit; Epididymis secretory sperm binding protein Li 99n ELISA Kit; ERp60 ELISA Kit; FLJ26680 ELISA Kit; grp60 ELISA Kit; HACBP ELISA Kit; HEL S 99n ELISA Kit; RO ELISA Kit; Sicca syndrome antigen A (autoantigen Ro; calreticulin) ELISA Kit; Sicca syndrome antigen A ELISA Kit; SSA ELISA Kit;Description:The product CSB-E09787h is a ready-to-use microwell, strip plate ELISA Kit for quantitatively detecting the amount of the Calreticulin (CRT/CALR) in human serum, plasma, urine, or tissue homogenates. This assay kit was designed and optimized for Tags & Cell Markers-related research in humans. It has undergone rigorous quality control in multiple parameters, including sensitivity, specificity, precision, linearity, recovery, and inter-batch difference. Refer to the product instructions for more details. This assay employs the quantitative sandwich enzyme immunoassay technique, in which CRT in the samples or standards are sandwiched between pre-coated CRT antibody and Biotin-conjugated CRT antibody. HRP-avidin is then added into the wells. Following a wash to remove any unbound reagent, the TMB substrate solution is added to the wells and color develops in proportion to the amount of CRT bound in the initial step. The color development is stopped upon adding the stop solution, and the intensity of the color is measured at 450 nm via a microplate reader. The levels of CRT in the samples can be determined by referring to the O.D. (optical density) of the samples to the standard curve. CRT, also called CALR, is an endoplasmic reticulum (ER) chaperone protein involved in conformation-dependent molecular sorting of newly synthesized proteins (known as quality control (QC)). Within the ER, CALR normally binds misfolded proteins and retains them for ER-associated degradation (ERAD). Outside of the ER, CALR also participates in a variety of biological processes, including antigen processing and presentation for the adaptive immune response, cell adhesion/migration, cell proliferation, and immunogenic cell death. In the nucleus, CALR regulates gene expression and influences cell proliferation by suppressing interactions between retinoic acid receptor and its DNA response elements.;Species Reactivity:Human;Usage:For Research Use Only;Detection Principle:Sandwich ELISA;Contents of Kit:A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human CRT antibody. This dismountable microplate can be divided into 12 x 8 strip plates. Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve. One vial Biotin-labeled CRT antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody. One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic. One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody. One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution. One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration. One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances. One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue. One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow. Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation. An instruction manual;Detection Range:0.156 ng/mL-10 ng/mL;Sensitivity:0.039 ng/mL;