Cat#:ADV-3438EK;Product Name:Human Annexin Ⅰ(ANX-Ⅰ) ELISA Kit;Size:96T, 5×96T, 10×96T;Sample:Serum, Plasma, Tissue Homogenates;Synonym:Annexin 1 ELISA Kit; Annexin A1 ELISA Kit; Annexin I (lipocortin I) ELISA Kit; Annexin I ELISA Kit; Annexin-1 ELISA Kit; AnnexinA1 ELISA Kit; AnnexinI ELISA Kit; ANX 1 ELISA Kit; ANX A1 ELISA Kit; ANX1 ELISA Kit; ANXA 1 ELISA Kit; ANXA1 ELISA Kit; ANXA1 protein ELISA Kit; ANXA1_HUMAN ELISA Kit; Calpactin 2 ELISA Kit; Calpactin II ELISA Kit; Calpactin-2 ELISA Kit; CalpactinII ELISA Kit; Chromobindin 9 ELISA Kit; Chromobindin-9 ELISA Kit; Chromobindin9 ELISA Kit; HGNC:533 ELISA Kit; Lipocortin 1 ELISA Kit; Lipocortin I ELISA Kit; Lipocortin1 ELISA Kit; LipocortinI ELISA Kit; LPC 1 ELISA Kit; LPC1 ELISA Kit; p35 ELISA Kit; Phospholipase A2 inhibitory protein ELISA Kit;Description:The ELISA Kit is designed for quantitatively measuring human ANXA1 levels in samples, including serum, plasma, or tissue homogenates. It uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of ANXA1 in samples. The color intensity is measured at 450 nm via a microplate reader. ANXA1 is a calcium-dependent phospholipid-binding protein with a phospholipase A2 inhibitory activity. ANXA1 exerts multiple roles in important biological processes such as cell differentiation, proliferation, plasma membrane repair, epithelial repair, and cell apoptosis. In normal health conditions, ANXA1 plays a major protective role in the brain through the promotion of blood-brain-barrier (BBB) integrity. Several studies have indicated a role for ANXA1 in neurological diseases, including Alzheimer’s disease (AD) and stroke. Studies have also shown that ANXA1 is involved in multiple anti-inflammatory processes, including regulation of neutrophil migration, macrophage phagocytosis, and induction of changes in cell polarity of microglial cells after ischemia-like injury in vitro.;Species Reactivity:Human;Usage:For Research Use Only;Detection Principle:Sandwich ELISA;Contents of Kit:A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human ANXA1 antibody.This dismountable microplate can be divided into 12 x 8 strip plates. Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve. One vial Biotin-labeled ANXA1 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody. One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic. One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody. One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution. One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration. One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances. One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue. One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow. Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation. An instruction manual;Detection Range:0.312 ng/mL-20 ng/mL;Sensitivity:0.078 ng/mL;