Cat#:EIA-447C;Product Name:Human MIF ELISA Kit;Size:96T;Sample:human serum, plasma and other biological fluids;Intended use:RUO;Synonym:MIF; macrophage migration inhibitory factor (glycosylation-inhibiting factor); GIF; GLIF; MMIF; Macrophage migration inhibitory factor; EC=5.3.2.1; Glycosylation-inhibiting factor; L-dopachrome isomerase; L-dopachrome tautomerase; EC=5.3.3.12; Phenylpyruvate tautomerase; macrophage migration inhibitory factor; OTTHUMP00000198465; phenylpyruvate tautomerase;Background:MIF is a proinflammatory cytokine involved in many inflammatory reactions and disorders. MIF (macrophage migration inhibitory factor) was one of the first cytokines to be discovered and was initially described as a T cell-derived factor that inhibits the random migration of macrophages (Weiser 1989). Recently, MIF was rediscovered as a pituitary hormone that act as the counterregulatory hormone for glucocorticoid action within the immune system (Bernhagen 1993, Mitchell 1995). MIF was released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secreted MIF counter-regulates the immunosuppressive effects of steroids on immune cell activation and cytokine production (Bucala 1998). MIF plays a critical role in the host control of inflammation and immunity. MIF is involved in both autoimmune disorders and tumorigenesis.;Species Reactivity:Human;Application:quantitative;Storage:Unopened Kit: Store at 2 - 8°C. Do not use past kit expiration date. Opened/Reconstituted Reagents: TMB Solution A; TMB Solution B; TMB Stop Solution; Wash Buffer; HRP-conjugate antibody The above mentioned reagents should be stored for up to 1 month at 2 - 8°C. Microplate Wells: Return unused wells to the foil pouch containing the desiccant pack, reseal along entire edge of zip-seal. May be stored for up to 1 month at 2 - 8°C.;Usage:For Lab Research Use Only;Detection Principle:Sandwish ELISA;