Cat#:EIA-432C;Product Name:Human OPN ELISA Kit;Size:96T;Sample:human serum, plasma, tissue homogenates, cell culture supernates and other biological fluids;Intended use:RUO;Synonym:SPP1; secreted phosphoprotein 1; OPN; BNSP; BSPI; ETA-1; bone sialoprotein I; osteopontin; early T-lymphocyte activation 1; Osteopontin; Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1; SPP-1; Urinary stone protein; Uropontin; uropontin; nephropontin; osteopontin-C; osteopontin-D; OTTHUMP00000161173; OTTHUMP00000161174; OTTHUMP00000218555; OTTHUMP00000218556; urinary stone protein; early T-lymphocyte activation 1; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1);Background:Osteopontin is the principal phosphorylated glycoprotein of bone and is expressed in a limited number of other tissues including dentine. Osteopontin is produced by osteoblasts under stimulation by calcitriol and binds tightly to hydroxyapatite. It is also involved in the anchoring of osteoclasts to the mineral of bone matrix via the vitronectin receptor, which has specificity for osteopontin. Osteopontin is overexpressed in a variety of cancers, including lung, breast, colorectal, stomach, ovarian, melanoma and mesothelioma.;Species Reactivity:Human;Application:quantitative;Storage:Unopened Kit: Store at 2 - 8°C. Do not use past kit expiration date. Opened/Reconstituted Reagents: TMB Solution A; TMB Solution B; TMB Stop Solution; Wash Buffer; HRP-conjugate antibody The above mentioned reagents should be stored for up to 1 month at 2 - 8°C. Microplate Wells: Return unused wells to the foil pouch containing the desiccant pack, reseal along entire edge of zip-seal. May be stored for up to 1 month at 2 - 8°C.;Usage:For Lab Research Use Only;Detection Principle:Sandwish ELISA;