Cat#:EIA-312C;Product Name:Mouse IgG ELISA Kit;Size:96T;Sample:serum or plasma;Intended use:RUO;Synonym:IgG; Ig gamma 1 chain C region; IGHG1; Immunoglobin heavy constant gamma 1; Immunoglobulin G;Background:There are five classes of mammalian immunoglobulins: IgA, IgD, IgE, IgM, and IgG. IgG is the most abundant immunoglobulin and is equally distributed in blood and tissue (Junqueira, Luiz C. and Jose Carneiro, 2003). In mice, the IgG class is further divided into four subclasses: IgG1, IgG2a/ IgG2c (strain specific), IgG2b, and IgG3. The general immunoglobulin structure is composed of four polypeptide chains, two heavy and two light chains linked together and to each other by disulfide bonds, creating a tetrameric quaternary structure. The resulting tetramer creates two identical halves which together form a Y like structure. IgG is primarily involved in the second class of antibodies to appear in response to a foreign antigen (IgM is the first). The presence of IgG usually signifies a mature antibody response (Meulenbroek, A.J and Zeijlemaker, W.P. 1996). IgG can bind to many pathogens and also plays an important role in antibody-dependent cell-mediated cytotoxicity. Mouse serum and plasma samples typically contain about 7 to 10 mg/ml of IgG.;Species Reactivity:Mouse;Application:quantitate;Storage:Unopened Kit: Store at 2 - 8°C. Do not use past kit expiration date. Opened/Reconstituted Reagents: TMB Solution A; TMB Solution B; TMB Stop Solution; Wash Buffer; HRP-conjugate antibody The above mentioned reagents should be stored for up to 1 month at 2 - 8°C. Microplate Wells: Return unused wells to the foil pouch containing the desiccant pack, reseal along entire edge of zip-seal. May be stored for up to 1 month at 2 - 8°C.;Usage:For Lab Research Use Only;Detection Principle:Sandwich ELISA;