Cat#:EIA-316C;Product Name:Mouse IgA ELISA Kit;Size:96 T;Sample:serum; plasma; milk; feces; saliva;Intended use:RUO;Synonym:immunoglobulin heavy constant alpha; IgA; Igh-2;Background:There are five classes of mammalian immunoglobulins: IgA;IgD; IgE; IgG; and IgM. In mice; the IgG class is further dividedinto four subclasses: IgG1; IgG2a/IgG2c (strain specific);IgG2b; and IgG3. IgA occurs as a single class in mice;whereas in humans; there are two subclasses: IgA1 and IgA2.IgA is the major immunoglobulin found in secretory fluidssuch as saliva; tears; and colostrum. Secretory IgA is also themost abundant immuoglobulin in the respiratory and intestinalmucosa where it serves as a first line of defense against bacterialand viral invasion from the external environment. SecretoryIgA is mainly formed as dimers and higher multimers thatare linked via a J-chain attached near the Fctermini. SecretoryIgA also contains a 70 kDa polypeptide associated withthe alpha heavy chain; the secretory component (SC); whichhas been shown to protect IgA molecules from protease activity.IgA is also present in the serum; but in a monomeric formwhere it functions mainly in the inflammatory response via itsinteractions with receptors specific for the IgA Fc region(FcalphaR) on effector immune cells. The reported concentrationof serum IgA in normal BALB/c mice is around 0.7mg/ml.;Species Reactivity:Mouse;Application:Quantitative;Storage:Unopened Kit: Store at 2 - 8°C. Do not use past kit expiration date. Opened/Reconstituted Reagents: TMB Solution A; TMB Solution B; TMB Stop Solution; Wash Buffer; HRP-conjugate antibody The above mentioned reagents should be stored for up to 1 month at 2 - 8°C. Microplate Wells: Return unused wells to the foil pouch containing the desiccant pack, reseal along entire edge of zip-seal. May be stored for up to 1 month at 2 - 8°C.;Usage:For Lab Research Use Only;Detection Principle:Sandwich ELISA;