Cat#:EIA-547C;Product Name:Human EDN1 ELISA Kit;Size:96T;Sample:SERUM, EDTA-PLASMA, URINE AND CELL CULTURE SUPERNATANTS;Intended use:N/A;Synonym:EDN1; endothelin 1; ET1; PPET1; HDLCQ7; endothelin-1; OTTHUMP00000016038; preproendothelin-1; Preproendothelin-1;Background:Big Endothelin-1 (Big ET), a small 38-amino-acid peptide, is the biological precursor of Endothelin (1-21), the most potent vasoconstrictor known today. Various cell types including vascular endothelial cells and non-vascular cells (eg. mesanglial, kidney and epithelial cells), produce Endothelin. Cleavage of Big ET by the Endothelin Converting Enzyme (ECE) leads to the active ET (1-21) and to the C-terminal fragment (22-38). The physiological importance of cleavage of Big ET is indicated by the reported 140-fold increase in vasoconstrictor activity upon cleavage to ET-1, although both peptides can be determined in about equimolar concentrations in plasma. It was demonstrated that the half-life of ET (1-21) in plasma is less than one minute, whereas clearance of Big ET is much slower.;Species Reactivity:Human;Application:QUANTITATIVE;Storage:Unopened Kit: Store at 2 - 8°C. Do not use past kit expiration date. Opened/Reconstituted Reagents: TMB Solution A; TMB Solution B; TMB Stop Solution; Wash Buffer; HRP-conjugate antibody The above mentioned reagents should be stored for up to 1 month at 2 - 8°C. Microplate Wells: Return unused wells to the foil pouch containing the desiccant pack, reseal along entire edge of zip-seal. May be stored for up to 1 month at 2 - 8°C.;Usage:For Lab Research Use Only;Detection Principle:Sandwich ELISA;
SERUM, EDTA-PLASMA, URINE AND CELL CULTURE SUPERNATANTS
Intended use:
N/A
Gene Introduction:
Big Endothelin-1 (Big ET), a small 38-amino-acid peptide, is the biological precursor of Endothelin (1-21), the most potent vasoconstrictor known today. Various cell types including vascular endothelial cells and non-vascular cells (eg. mesanglial, kidney and epithelial cells), produce Endothelin. Cleavage of Big ET by the Endothelin Converting Enzyme (ECE) leads to the active ET (1-21) and to the C-terminal fragment (22-38). The physiological importance of cleavage of Big ET is indicated by the reported 140-fold increase in vasoconstrictor activity upon cleavage to ET-1, although both peptides can be determined in about equimolar concentrations in plasma. It was demonstrated that the half-life of ET (1-21) in plasma is less than one minute, whereas clearance of Big ET is much slower.
Species Reactivity:
Human
Application:
QUANTITATIVE
Usage:
For Lab Research Use Only
Detection Principle:
Sandwich ELISA
Storage:
Unopened Kit: Store at 2 - 8°C. Do not use past kit expiration date. Opened/Reconstituted Reagents: TMB Solution A; TMB Solution B; TMB Stop Solution; Wash Buffer; HRP-conjugate antibody The above mentioned reagents should be stored for up to 1 month at 2 - 8°C. Microplate Wells: Return unused wells to the foil pouch containing the desiccant pack, reseal along entire edge of zip-seal. May be stored for up to 1 month at 2 - 8°C.