Cat#:EIA-554C;Product Name:Human CTSK ELISA Kit;Size:96 T;Sample:SERUM OR CELL CULTURE;Intended use:RUO;Synonym:CTSK; cathepsin K; CTSO; PKND; PYCD; CTS02; CTSO1; CTSO2; MGC23107; cathepsin K; cathepsin X; cathepsin O1; cathepsin O2; EC 3.4.22.38; cathepsin K (pycnodysostosis); OTTHUMP00000032938; Cathepsin O;Background:Cathepsin K is a highly expressed cystein protease, secreted by osteoclasts. It is produced as a 329 AA pre-cursor, which is cleaved to its active form with a length of 215 AA (MW 23,495) by osteoclasts, when they are resorbing bone. Cathepsin K degrades the collagen matrix of the bone after removal of its mineral components. It is actually the most abundantly synthesised protein of the active, resorbing osteoclast and therefore the most specific marker of resorption activity. Cathepsin K plays a key role in tissue destruction, remodelling and cartilage breakdown of the bone. The high homology of Cathepsin K across various species (mouse 86%, rat 88%, pig 97%, rabbit 96%) should allow the use of this assay for studies in animal models for following diseases;Species Reactivity:Human;Application:Quantitative;Storage:Unopened Kit: Store at 2 - 8°C. Do not use past kit expiration date. Opened/Reconstituted Reagents: TMB Solution A; TMB Solution B; TMB Stop Solution; Wash Buffer; HRP-conjugate antibody The above mentioned reagents should be stored for up to 1 month at 2 - 8°C. Microplate Wells: Return unused wells to the foil pouch containing the desiccant pack, reseal along entire edge of zip-seal. May be stored for up to 1 month at 2 - 8°C.;Usage:For Lab Research Use Only;Detection Principle:Sandwich ELISA;