Cat#:EIA-640C;Product Name:HIA IgG ELISA Kit;Size:96wells;Sample:human plasma;Intended use:in vitro diagnostic;Synonym:heparin-dependent antibodies; heparin; unfractionated hepari; glycosaminoglycan; anticoagulant; HIA;Background:Heparin dependent antibodies are immunoglobulins present in plasma of patients with suspicion of Heparin-Induced Thrombocytopenia (HIT) type II. Type II HIT, the immunoallergic type, occurs during heparin treatment and remains a major complication of this therapy. It is caused by the development of antibodies to Heparin-Protein (usually Platelet Factor 4) macromolecular complexes. In addition to antibodies to PF4-Heparin, antibodies to other chemokines such as Neutrophil- Activating Peptide or NAP2 and Interleukin-8 or IL8 have also been evidenced in some patients. Development of pathology is mainly associated with heparin-dependent antibodies of the IgG isotype. However, when the test is used for assessing the risk of developing a clinical complication of HIT, the assay of the global IgGAM isotypes is useful as a prognostic factor for this complication. When HIT occurs first, inflammation and/or platelet activation mechanisms, associated with various medical or surgical contexts, develop and lead to an increased release of chemokines and then promote formation of heparin complexes with chemokines (usually PF4). These multimolecular complexes can become antigenic and induce the generation of heparin-dependent antibodies. Heterogeneity of these antibodies could partly explain some discrepancies between the clinical suspicion of HIT and biological tests [6]. Frequently, heparin dependent antibodies can be asymptomatic, especially when they are of the IgM isotype. The clinical association is higher with elevated antibody concentrations and with the IgG isotype.;Species Reactivity:Human;Application:Qualitative;Storage:Unopened Kit: Store at 2 - 8°C. Do not use past kit expiration date. Opened/Reconstituted Reagents: TMB Solution A; TMB Solution B; TMB Stop Solution; Wash Buffer; HRP-conjugate antibody The above mentioned reagents should be stored for up to 1 month at 2 - 8°C. Microplate Wells: Return unused wells to the foil pouch containing the desiccant pack, reseal along entire edge of zip-seal. May be stored for up to 1 month at 2 - 8°C.;Usage:For Lab Research Use Only;Detection Principle:I-ELISA;