Cat#:EIA-667C;Product Name:H5 Influenza DOT-ELISA Kit;Size:6T/30T;Sample:avian excretes, human nasopharyngeal aspirates, swabs, nasal wash, chicken embryo whole virus inoculation or viral lysates, etc.;Intended use:in vitro diagnostic;Synonym:H5 Influenza; H5HA; Hemagglutinin 5; Avian influenza; highly pathogenic avian influenza;Background:Avian influenza (AI) is an infectious disease of birds caused by type A strains of the influenza virus (AIV). The disease, first identified in Italy more than 100 years ago, now occurs worldwide. Infection triggers a wide spectrum of symptoms in birds, ranging from mild illnesses to a highly contagious and rapidly fatal disease resulting in severe epidemics. In the (caused by H5N1-strain of AIV and also called highly pathogenic avian influenza) outbreak in Hong Kong in 1997, patients had developed symptoms of fever, sore throat, cough and, in several of the fatal cases, severe respiratory distress secondary to viral pneumonia. Previously healthy adults and children, and some of whom had chronic medical conditions, were affected. More recently, outbreaks of avian influenza caused by the H5N1 strain occurred among poultry in eight countries in Asia (Cambodia, China, Indonesia, Japan, Laos, South Korea, Thailand, and Vietnam) during late 2003 and early 2004. At that time, more than 100 million birds in the affected countries either died from the disease or were killed in order to try to control the outbreak. By March 2004, the outbreak was reported to be under control. Beginning in late June 2004, however, new deadly outbreaks of AIV H5N1 among poultry were reported by several countries in Asia (Cambodia, China, Indonesia, Malaysia [first-time reports], Thailand, and Vietnam ). It is believed that these outbreaks are ongoing. Human infections with AIV H5N1 strain, however, have been reported in Cambodia (1case/1 death), Thailand (17cases/1 deaths) and Vietnam (51cases/ 33deaths) during both of these outbreak periods. Hemagglutinin (HA) is a surface glycoprotein on Influenza A responsible for binding to N-AcetylNeuraminic Acid (NeuNAc) or commonly Sialic acid on host cell surface receptors. The Influenza viruses from the A virus group have principally similar morphological, chemical and biological features. The differentiation of the types is possible by the different antigenicity of their nucleo- and matrix proteins that have type-specific antigenicity. However, the essential immunodominant antigens and primary targets in diagnosis are the hemagglutinin (HA) and the neuraminidase (NA) antigens.;Species Reactivity:Virus;Application:Qualitative;Storage:Unopened Kit: Store at 2 - 8°C. Do not use past kit expiration date. Opened/Reconstituted Reagents: TMB Solution A; TMB Solution B; TMB Stop Solution; Wash Buffer; HRP-conjugate antibody The above mentioned reagents should be stored for up to 1 month at 2 - 8°C. Microplate Wells: Return unused wells to the foil pouch containing the desiccant pack, reseal along entire edge of zip-seal. May be stored for up to 1 month at 2 - 8°C.;Usage:For Lab Research Use Only;Detection Principle:Sandwich ELISA;