Cat#:EIA-703C;Product Name:Dog IgG ELISA Kit;Size:96T;Sample:serum, plasma, colostrum and milk;Intended use:RUO;Synonym:IgG; Ig gamma 1 chain C region; IGHG1; Immunoglobin heavy constant gamma 1; Immunoglobulin G;Background:In mammalian species immunoglobulin G (IgG) is usually the most abundant immunoglobulin isotype in serum. The concentration of total IgG in the plasma of normal, healthy dogs range roughly from 10 -20 mg/ml. In colostrum, it usually starts at about 15 mg/ml and gradually decreases to a stable level of about 1-2 mg/ml after one week. The IgG concentration in dog urine is normally very low (<1 μg/ml). The IgG concentration in these biological fluids is relatively stable for a particular individual. However, significant changes in IgG concentrations either relative to other immunoglobulin isotypes, or to the relative ratios of the different IgG sub-classes, are usually associated with infection or disease. For example, elevated levels of IgG have been associated with the onset of visceral leishmaniasis (VL), gingivitis, dermatitis, staphylococcal pyoderma, stress, dehydration, blood diseases, liver disease, heart disease, various allergies, diabetes and arthritis. Dogs are also sometimes used as animal models for pharmacological studies. Therefore, it is important to have an accurate quantitation of total IgG in their plasma and serum.;Species Reactivity:Dog;Application:quantitate;Storage:Unopened Kit: Store at 2 - 8°C. Do not use past kit expiration date. Opened/Reconstituted Reagents: TMB Solution A; TMB Solution B; TMB Stop Solution; Wash Buffer; HRP-conjugate antibody The above mentioned reagents should be stored for up to 1 month at 2 - 8°C. Microplate Wells: Return unused wells to the foil pouch containing the desiccant pack, reseal along entire edge of zip-seal. May be stored for up to 1 month at 2 - 8°C.;Usage:For Lab Research Use Only;Detection Principle:Sandwich ELISA;