Cat#:ADV-0783EK;Product Name:Rat Immunoglobulin G, IgG ELISA Kit;Size:96T, 5×96T, 10×96T;Sample:Serum, Plasma;Description:The rat immunoglobulin G (IgG) ELISA kit is employed for the quantitative analysis of IgG levels in rat samples. This kit is specifically designed for rat species and is suitable for testing serum and plasma samples. With a detection range of 31.25 ng/mL to 8000 ng/mL and a sensitivity of 31.25 ng/mL, this kit provides both broad detection capabilities and high sensitivity. The kit employs a competitive measurement method, ensuring accurate results. The assay time ranges from 1 to 5 hours, making it efficient for laboratory use. A small sample volume of 50-100 µl is required for analysis, and the detection wavelength is set at 450 nm. The kit has been utilized in several research studies, indicating its reliability and effectiveness in scientific investigations.;Species Reactivity:Rat;Usage:For Research Use Only;Detection Principle:Competitive ELISA;Contents of Kit:A 96-well Assay plate --The 96-well plate has been pre-coated with rat IgG. Standard (6 x 0.5 ml)--Dilute the standard at dilution series, read the OD values, and then draw a standard curve. HRP-conjugated IgG(1 x 6 ml) --Bind to the IgG, and HRP catalyzes the TMB to elicit a chromogenic reaction. Sample Diluent (2 x 20 ml) --Reconstitute the standard and dilute the sample to an appropriate concentration. Wash Buffer (25x concentrate) 1 x 20 ml --Wash away unbound or free substances. TMB Substrate (1x 10 ml) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue. Stop Solution (1 x 10ml) --Stop the color reaction. The solution color immediately turns from blue to yellow. Four Adhesive Strips (For 96 wells) An Instruction manual;Detection Range:31.25 ng/mL-8000 ng/mL;Sensitivity:31.25 ng/mL;