Cat#:ADV-1368EK;Product Name:Rat 5-Hydroxytryptamine, 5HT ELISA Kit;Size:96T, 5×96T, 10×96T;Sample:Serum, Plasma, Cell Lysates, Tissue Homogenates;Description:This Rat 5HT ELISA Kit was designed for the quantitative measurement of Rat 5HT protein in serum, plasma, cell lysates, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.8 ng/mL-160 ng/mL and the sensitivity is 0.4 ng/mL.;Species Reactivity:Rat;Usage:For Research Use Only;Detection Principle:Competitive ELISA;Contents of Kit:A 96-well Assay plate -- The 96-well plate has been pre-coated with goat-anti-rabbit 5HT antibody. Standard 6 x 0.5 ml -- Dilute the standard at dilution series, read the OD values, and then draw a standard curve. Anti-rat 5HT Antibody 1 x 6 ml -- Act as the detection antibody. HRP-conjugated 5HT 1 x 6 ml -- Bind to the 5HT antibody, and HRP catalyzes the substrate A and B to elicit a chromogenic reaction. Wash Buffer (20 x concentrate) 1 x 15 ml -- Wash away unbound or free substances. Substrate A 1 x 7 ml -- Mix with substrate B, and the TMB is catalyzed by HRP to develop color. Substrate B 1 x 7 ml -- Mix with substrate A, and the TMB is catalyzed by HRP to develop color. Stop Solution 1 x 7 ml -- Stop the color reaction. The solution color immediately turns from blue to yellow. Four Adhesive Strips (For 96 wells) An Instruction manual;Detection Range:0.8 ng/mL-160 ng/mL;Sensitivity:0.4 ng/mL;