Cat#:ADV-0198EK;Product Name:Mouse thyroxine, T4 ELISA Kit;Size:96T, 5×96T, 10×96T;Sample:Serum, Plasma, Tissue Homogenates;Description:This Mouse thyroxine (T4) ELISA Kit was designed for the quantitative measurement of Mouse thyroxine (T4) protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 20 ng/mL-320 ng/mL and the sensitivity is 20 ng/mL.;Species Reactivity:Mouse;Usage:For Research Use Only;Detection Principle:Competitive ELISA;Contents of Kit:A 96-well Assay plate -- The 96-well plate has been pre-coated with anti-mouse T4 antibody. Standard 6 x 1 ml -- Dilute the standard at dilution series, read the OD values, and then draw a standard curve. Biotin-conjugated T4 1 x 6 ml HRP-avidin 1 x 6 ml -- HRP catalyzes the TMB to elicit a chromogenic reaction. Wash Buffer (20 x concentrate) 1 x 15 ml -- Wash away unbound or free substances. Substrate A 1 x 7 ml -- Mix with substrate B, and the TMB is catalyzed by HRP to develop color. Substrate B 1 x 7 ml -- Mix with substrate A, and the TMB is catalyzed by HRP to develop color. Stop Solution 1 x 7 ml -- Stop the color reaction. The solution color immediately turns from blue to yellow. Four Adhesive Strips (For 96 wells) An Instruction manual;Detection Range:20 ng/mL-320 ng/mL;Sensitivity:20 ng/mL;
This Mouse thyroxine (T4) ELISA Kit was designed for the quantitative measurement of Mouse thyroxine (T4) protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 20 ng/mL-320 ng/mL and the sensitivity is 20 ng/mL.
Species Reactivity:
Mouse
Usage:
For Research Use Only
Detection Principle:
Competitive ELISA
Contents of Kit:
A 96-well Assay plate -- The 96-well plate has been pre-coated with anti-mouse T4 antibody. Standard 6 x 1 ml -- Dilute the standard at dilution series, read the OD values, and then draw a standard curve. Biotin-conjugated T4 1 x 6 ml HRP-avidin 1 x 6 ml -- HRP catalyzes the TMB to elicit a chromogenic reaction. Wash Buffer (20 x concentrate) 1 x 15 ml -- Wash away unbound or free substances. Substrate A 1 x 7 ml -- Mix with substrate B, and the TMB is catalyzed by HRP to develop color. Substrate B 1 x 7 ml -- Mix with substrate A, and the TMB is catalyzed by HRP to develop color. Stop Solution 1 x 7 ml -- Stop the color reaction. The solution color immediately turns from blue to yellow. Four Adhesive Strips (For 96 wells) An Instruction manual