Cat#:ADV-1435EK;Product Name:Mouse Retinoic Acid ELISA kit;Size:96T, 5×96T, 10×96T;Sample:Serum, Plasma;Description:This Mouse Retinoic Acid ELISA Kit was designed for the quantitative measurement of Mouse Retinoic Acid protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 25 pg/mL-400 pg/mL and the sensitivity is 12.5 pg/mL.;Species Reactivity:Mouse;Usage:For Research Use Only;Detection Principle:Competitive ELISA;Contents of Kit:An assay plate --- The 96-well plate has been pre-coated with the anti-mouse retinoic acid antibody. Lyophilized standard (1 x 200 μl) --- Reconstitute the standard with sample diluent at dilution series and draw the standard curve. HRP-conjugate retinoic acid (100 x concentrate) (1 x 60 μl)--- HRP catalyzes the TMB substrate for color development and needs to be diluted before use. HRP-avidin Diluent (1 x 10 ml) --- Dilute the HRP-avidin. Sample Diluent (2 x 20 ml) --- Reconstitute the standard and dilute the sample to an appropriate concentration. Wash Buffer (25 x concentrate) (1 x 20 ml) --- Wash away the unbound solution and non-specific substances. TMB Substrate --- TMB interacts with HRP, eliciting the solution turns blue. Stop Solution (1 x 10 ml) --- Add stop solution and stop the color development. The solution color immediately turns from blue to yellow. Four Adhesive Strips (For 96 wells) --- Cover the microtiter plate when incubation. An instruction manual;Detection Range:25 pg/mL-400 pg/mL;Sensitivity:12.5 pg/mL;