Cat#:ADV-1053EK;Product Name:Mouse estrogen, E ELISA Kit;Size:96T, 5×96T, 10×96T;Sample:Serum, Plasma;Description:This Mouse estrogen (E) ELISA Kit was designed for the quantitative measurement of Mouse estrogen (E) protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 25 pg/mL-1000 pg/mL and the sensitivity is 25 pg/mL.;Species Reactivity:Mouse;Usage:For Research Use Only;Detection Principle:Competitive ELISA;Contents of Kit:A 96-well Assay plate--The 96-well plate has been pre-coated with goat-anti-rabbit estrogen antibody. Standard (6 x 0.5 ml)--Dilute the standard at dilution series, read the OD values, and then draw a standard curve. HRP-conjugated estrogen(1 x 6 ml) --Bind to the anti-mouse estrogen antibody, and HRP catalyzes the TMB to elicit a chromogenic reaction. Anti-mouse estrogen Antibody (1 x 6 ml)--React with both estrogen in the samples/standards and HRP-conjugated estrogen. Wash Buffer (20x concentrate) 1 x 15ml --Wash away unbound or free substances. Substrate A (1 x 7 ml) --Mix with Substrate B and interacts with HRP, eliciting the solution turns blue. Substrate B (1 x 7 ml) --Mix with Substrate A and interacts with HRP, eliciting the solution turns blue. Stop Solution (1 x 7ml) --Stop the color reaction. The solution color immediately turns from blue to yellow. Four Adhesive Strips (For 96 wells) An Instruction manual;Detection Range:25 pg/mL-1000 pg/mL;Sensitivity:25 pg/mL;