Cat#:ADV-0098EK;Product Name:Human Vitamin B12, VB12 ELISA Kit;Size:96T, 5×96T, 10×96T;Sample:Serum, Plasma;Description:The human Vitamin B12 (VB12) ELISA kit is a quantitative assay designed for detecting Vitamin B12 levels specifically in human serum and plasma samples. This ELISA kit has a detection range of 1.56 pg/mL to 100 pg/mL with a sensitivity of 1.498 pg/mL, making it highly accurate in measuring Vitamin B12 concentrations. This content looks too robotic The assay principle is competitive, and the detection wavelength is at 450 nm. The assay can be completed within 1 to 5 hours, requiring a sample volume of 50-100 µl. This kit has been utilized in more than 4 research papers, showcasing its reliability and effectiveness in Vitamin B12 analysis.;Species Reactivity:Human;Usage:For Research Use Only;Detection Principle:Competitive ELISA;Contents of Kit:A 96-well Assay plate --The 96-well plate has been pre-coated with anti-human VB12 antibody. Standard (2 x 0.5 ml)--Dilute the standard at dilution series, read the OD values, and then draw a standard curve. Biotin-conjugated VB12(100 x concentrate) (1 x 600 μl) HRP-avidin (100 x concentrate) (1 x 120 μl) Biotin-conjugate Diluent (1 x 10 ml) --Dilute the Biotin-conjugated VB12 HRP-avidin Diluent (1 x 20 ml) --Dilute the HRP-avidin solution. Sample Diluent (2 x 20 ml) --Reconstitute the standard and dilute the sample to an appropriate concentration. Wash Buffer (25x concentrate) (1 x 20 ml)--Wash away unbound or free substances. TMB Substrate (1x 10 ml) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue. Stop Solution (1 x 10ml) --Stop the color reaction. The solution color immediately turns from blue to yellow. Four Adhesive Strips (For 96 wells) An Instruction manual;Detection Range:1.56 pg/mL-100 pg/mL;Sensitivity:1.498 pg/mL;