Cat#:ADV-0855EK;Product Name:Human heparan sulfate, HS ELISA Kit;Size:96T, 5×96T, 10×96T;Sample:Serum, Plasma, Urine, Tissue Homogenates;Description:This Human heparan sulfate (HS) ELISA Kit was designed for the quantitative measurement of Human heparan sulfate (HS) protein in serum, plasma, urine, tissue homogenates. It is a Competitive ELISA kit, its detection range is 31.25 ng/mL-8000 ng/mL and the sensitivity is 31.25 ng/mL.;Species Reactivity:Human;Usage:For Research Use Only;Detection Principle:Competitive ELISA;Contents of Kit:A 96-well Assay plate --The 96-well plate has been pre-coated with HS. Standard (2 x 250 μl)--Dilute the standard at dilution series, read the OD values, and then draw a standard curve. Anti-human HS Antibody (100 x concentrate) (1 x 60 μl) Antibody Diluent (1 x 10 ml) --Dilute the anti-human LRG1 HRP-conjugated goat-anti-mouse HS antibody (100 x concentrate) (1 x 120 μl) --Act as the detection antibody. HRP-conjugate Diluent (1 x 20 ml) --Dilute the HRP-conjugated HS antibody. Sample Diluent (2 x 20 ml) --Reconstitute the standard and dilute the sample to an appropriate concentration. Wash Buffer (25x concentrate) (1 x 20 ml)--Wash away unbound or free substances. TMB Substrate (1x 10 ml) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue. Stop Solution (1 x 10ml) --Stop the color reaction. The solution color immediately turns from blue to yellow. Four Adhesive Strips (For 96 wells) An Instruction manual;Detection Range:31.25 ng/mL-8000 ng/mL;Sensitivity:31.25 ng/mL;